Equipments

From development to pre-clinical production, MTInov has a set of dedicated premises and equipment. Experimental research and modelling complement each other to better characterise cultures and improve the quality and quantity of cells and biomolecules of interest produced.


 

Premises

The MTInov platform consists of a laboratory adjoining the classified Cell Therapy laboratories.

This MTI laboratory has its own SAS and ventilated counters. It is an ISO 7 class room equipped with two GMP platforms: an isolator and a Prodigy system. A cryopreservation room allows the storage of cryopreserved products in conditions that comply with current safety standards.

For preclinical studies, a 100 m² clean room with overpressure (Iso6), with an airlock and dedicated to the culture of animal and human cells is available. It is equipped with culture systems from the microplate scale to 2L bioreactors.

An analysis room (50 m²), a molecular biology room (15 m²) and a microscopy room (10 m²) are located in the vicinity of this clean room. The ENSAIA’s Structural Analysis and Metabolomics platform (200 m²) provides the platform with recent tools dedicated to the analysis of biomolecules, while integrating solutions for the preparation and purification of samples.

Equipments

The MTI laboratory is equipped with a custom-made isolator dedicated to the GMP culture of adherent or suspension cells. Equipped with hypoxic incubators, an inverted microscope, a centrifuge and all the small equipment necessary for cell culture, it allows us to offer class A production from the entry of the raw material to the exit of the finished product.

The Prodigy, also installed in the MTI laboratory, is a GMP platform for immunomagnetic separation. It is equipped with a thermostatically controlled centrifuge/incubator allowing complete processes to be carried out. This platform is particularly suitable for the production of CAR-T cells.

Instrumented production stations in mechanically stirred bioreactors are available for on-line monitoring, scale-up and bioprocess optimisation studies:

  • Six 50-500 mL autoclavable mini bioreactors (GPC).
  • Two 0.5-2 L autoclavable bioreactors (Pierre Guérin).
  • One 0.5-1.5 L bioreactor (Solaris Biotech) with single-use bags (CerCell).
  • An instrumented platform equipped with 4 single-use 100-250 mL vessels (to come, in the process of being ordered).
  • A 0.5-2L bioreactor with a perfusion system (to come, in the process of being ordered).

The on-line probes used are dielectric, Raman and near infrared spectroscopy probes. Off-line monitoring of the cultures is carried out by Vi-Cell, NuceloCounter, Cytonote, flow cytometry, Gallery analyser, optical and fluorescence microscopy, AFM, SEM, UHPLC-MS/MS and Maldi Tof-Tof.

Multi-scale modelling

Several scales are characterised and reconciled:

  • Process simulation: SuperProDesigner.
  • Flow simulation (CFD): ANSYS Fluent and CAD software, meshing and associated post-processing.
  • Modelling of phenomena by dimensional analysis (e.g. prediction of the correct suspension of microcarriers).
  • Modelling of kinetics and chemometrics: MATLAB and PLS_Toolbox.
  • Meso-modelling (cell-support interactions): Material studio.
  • Metabolic modelling: MATLAB and dedicated Toolbox.
  • Molecular modelling: Discovery studio.